STANDARD OPERATING PROCEDURE (SOP): PREPARATION OF CuSO4 SOLUTION

                                        BLOOD CENTRE NAME

1. SCOPE AND APPLICATION

The specific gravity of 1.053 is equivalent to 12.5 g/dl haemoglobin. Hence CuSO4 solution of specific gravity 1.053 is used for predonation haemoglobin test.

2. RESPONSIBILITY

    The technician/laboratory assistant in the donor area.

3. REFERENCE

• Standard Operating Procedures for Blood Transfusion Service, World Health Organization, New Delhi

• Transfusion Medicine Technical Manual, 2nd ed. Directorate General Health Services Ministry of Health & Family Welfare Government of India.

4. PROCEDURE

Stock solution is made as follows and kept in a jar or bottle.

Method -1 Preparation of CuSO 4 Solution

1. Dissolve 170 gm crystalline CuSO4.5H2O in 1000 ml distilled water( Working solution) at 25°C.

2. Every morning prepare fresh solution.

3. Add 51 ml stock solution to 49 ml distilled water.

4. Check Specific Gravity which should be 1.053. if not, adjust it using either stock solution or distilled water.

Method-2 

Dissolve 8.33 gm of pure air dried crystals of copper sulphate (CuSO4 5H2 O) in 100 ml of distilled water. The Specific gravity of the solution must be 1.053.

5. DOCUMENTATION

Record the volume of stock and working solution prepared on the register .