STANDARD OPERATING PROCEDURE (SOP)  : Estimation of Hemoglobin by Hemo-Control

                                        BLOOD CENTRE NAME

1.SCOPE AND APPLICATION

Testing of Haemoglobin by Hemo-Control photometer for those donors who fail in copper sulphate.

2.RESPONSIBILITY

It is the responsibility of the doctor on duty /technician working in the donor area.

3.MATERIALS REQUIRED

• Hemo-Control photometer.
• Microcuvettes.
•  Control cuvette.
•  Sterile gauze/cotton, spirit.

Method

i. Take out microcuvette from the container and close it again.

ii. Make the donor sit comfortably. Lightly massage the fingers to stimulate the circulation.

iii. The fingertip is cleaned thoroughly with a spirit swab and allowed to dry.

iv. The finger is punctured firmly near the tip with a sterile disposable lancet. 

v. The first drop of blood is wiped and 2^nd drop of blood once large enough, hold the tip of the microcuvette in the middle of the drop of blood and let the cavity fill in one step.

vi. Remove the surplus blood from outside of the microcuvette to avoid the contamination of the cuvette holder.

vii. Insert the microcuvette into the cuvette holder.

viii. Push lightly on the cuvette holder until it closes itself.

ix. Read off the results.

xii. Open the cuvette holder; take out the used cuvette and dispose of this in the correct way.

INTERPRETATION

The donors having hemoglobin 12.5 gm/dl or more are accepted for blood donation. In case the hemoglobin is lower than 12.5 g/dl, hematinics are prescribed to build up Hb.

The results are entered in the Donor Questionnaire and Informed Consent Form/Donor Register.

Note

The microcuvettes are used only once and are sensitive to moisture. Therefore, the microcuvettes are to be taken out only immediately before use.

Reading of the microcuvettes is taken immediately or within 10 minutes at the latest.