STANDARD OPERATING PROCEDURE (SOP)  :Quality Control Of CuSO4 solution

                                        BLOOD CENTRE NAME

SOP Number	Effective date	Pages	Author	Authorised by
Version	Review Period	No. of copies	Reviewed by	Date

LOCATION    Donor Room	SUBJECT   Copper Sulphate Solution
FUNCTION           Quality control	DISTRIBUTION – Quality Control Laboratory Supervisor – Master File

1. SCOPE AND APPLICATION

Copper sulphate solution is used for screening blood donors by testing the haemoglobin concentration before blood donation.

Copper sulphate solution is checked to ensure that a drop of blood sample of predetermined haemoglobin value reacts as expected ( sinks/floats).

2. RESPONSIBILITY

It is the responsibility of the Quality Control personnel to ensure testing of the reagent before use.

3. REFERENCES

Technical Manual of American Association of Blood Banks,20^th ed.,2020

4. MATERIALS REQUIRED

Equipment:

• Urinometer

Reagents:

• Copper sulphate working solution 
• Distilled water 
• EDTA blood samples of known haemoglobin concentration

Glassware:

• Coplin jar 
• Heparinised capillaries 

Miscellaneous:

• Tissue paper 
• Copper sulphate record book 
• Tube racks

5. PROCEDURE

Check the copper sulphate solution against a light source for the presence of precipitate/cloudiness.

↓

Check the specific gravity of the solution using a urinometer

↓

Copper sulphate being a coloured solution, the marking of the urinometer corresponding to the upper meniscus of the solution should be 1.053=12.5g% of haemoglobin

↓

Arrange the blood samples according to haemoglobin concentration in a rack

↓

Obtain samples of known Hb values

↓

Transfer 30ml copper sulphate working solution in a Coplin jar

↓

Mix the blood sample of known haemoglobin concentration by inversion 

↓

Fill heparinised capillary upto ¾ capacity with the blood sample

↓

Allow the drop of blood to fall gently into the copper sulphate solution

↓

Repeat the procedure for all the blood samples

↓

Note the result

↓

Record the results in the copper sulphate record book.

6. RESULTS

If the solution appears cloudy or precipitate is present, discarded.

The result of testing the solution is interpreted as follows:

	RESULT	Hb CONCENTRATION	INTERPRETATION
(a)	Blood drops floats	Hb<12.5g%	Fail (F)
(b)	Blood drops sinks	Hb<12.5g%	Pass (P)
(c)	Blood drops sinks slowly or Blood drop hesitates midway and sinks slowly	Hb<12.5g%	Pass fail reaction (P/F)